Primer Mix including an Internal Control (QS), Reaction Mix, Control DNA, DNA Size Standard 550 (ROX), Allelic Ladder Triplex AFS QS, and Nuclease-Free Water
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Product Details
The Investigator HDplex Kit amplifies 12 autosomal STR markers, most of which are not featured in commonly used marker standards. This enables the reliable differentiation of forensic samples derived from related individuals and gives heightened discriminatory power in these demanding cases. The genetic analysis is calibrated with BT5 Matrix.
The Investigator Triplex AFS QS Kit is for forensic stain pre-selection, which serves as a starting point for genotyping strategies. It enables simultaneous amplification of STR loci FGA (FIBRA), SE33 (ACTBP2) and Amelogenin from forensic or reference samples. The genetic analysis is calibrated with the DS-30 matrix.
Performance
Investigator HDplex and Triplex AFS QS Kits have been validated using the GeneAmp 9700 thermal cycler and the Applied Biosystems 3130 Genetic Analyzer.
The recommended amount of DNA under standard conditions is 0.35 ng. Internal validations using the Investigator HDplex Kit demonstrated robust and balanced results with 0.2–1.0 ng DNA, and reliable results with <0.1 ng DNA. Internal validations using the Investigator Triplex AFS QS Kit demonstrated robust and balanced results with 0.1–1.0 ng DNA, and reliable results with <0.1 ng DNA.