Enzyme reagent kit QIAseq

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For 24 reactions on Ion Torrent sequencers: Includes enzyme and buffer master mix for cfDNA library prep, Ion Torrent Adapter Plate 24-plex, Ion Torrent Library Amplification Primer and PCR Master Mix ✓ 24/7 automatic processing of online orders ✓ Knowledgeable and professional Product & Technical Support ✓ Fast and reliable (re)-ordering Features Optimal conversion of cfDNA at every step from plasma to NGS library through highly efficient ligation chemistry Go directly from eluant to library prep without quantification using a protocol supporting the widest range of cfDNA input (1–100ng) Generate PCR-free libraries from 10 ng of cfDNA Minimize PCR bias by the use of high-fidelity amplification reagents Reduce cross-contamination risk with pre-dispensed plate-based adapters for up to 96 samples/NGS run Product Details QIAseq cfDNA All-in-One Kits provide an optimized solution for cfDNA analysis using any NGS platform from Illumina or Ion Torrent. Kits enable optimal conversion of cfDNA at every step – from plasma to NGS library – and allow sensitive detection of even the rarest variants, providing you with the highest confidence in your results. Performance Designed for cfDNA The QIAseq cfDNA All-in-One protocol is specially optimized for cfDNA and generates sequencer-ready NGS libraries from plasma in less than 3 h (see figure " Designed for any NGS-based cfDNA research"). It uniquely combines cfDNA extraction from plasma with library preparation chemistry in a way that avoids sample loss, while ensuring optimal sample conversion at every step. This results in very uniform whole genome or whole exome coverage (see figure " Highly uniform coverage").