RNA purification reagent kit miRNeasy

solutionfor histology

miRNeasy Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease. Features Effective microRNA (miRNA) and total RNA purification, even from small samples Efficient enrichment of miRNA and RNAs 200 nt) fraction can be purified separately (for separate purification, an RNeasy MinElute Cleanup Kit is required when using the miRNeasy Micro or Mini Kit and an additional RNeasy 96 plate when using the miRNeasy 96 Kit). Purification of 1–12 samples can be automated on the QIAcube Connect. High-throughput processing in convenient 96-well format can be performed using centrifugation or vacuum procedures. Performance QIAzol based microRNA purification (miRNA purification) using miRNeasy Kits efficiently purifies RNA from small tissue (up to 5 mg tissue) and cell (up to 1 x 106 cells) samples RNA can be purified from a variety of tissues and cells, including difficult-to-lyse tissues. Purification of total RNA including miRNA allows direct comparison of miRNA expression levels with those of housekeeping reference genes or any other mRNA of interest. Alternatively, for some sensitive downstream applications, an miRNA-enriched fraction without larger RNA may be required. miRNeasy Kits enable efficient enrichment of RNA down to approximately 18 nt in size. Principle miRNeasy Kits combine phenol/guanidine-based lysis of samples with silica membrane-based purification of total RNA. QIAzol Lysis Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate lysis of tissues, to inhibit RNases,