Enzyme reagent kit QIAquick

for DNA extractionfor DNA cleanupfor biological samples

For gel extraction/cleanup of up to 10 μg DNA (70 bp to 10 kb) from enzymatic reactions Features Up to 95% recovery of ready-to-use DNA Fast and convenient procedure Cleanup of DNA up to 10 kb in three easy steps Gel loading dye for convenient sample analysis Product Details The QIAquick Gel Extraction Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments from gels (up to 400 mg slices) or enzymatic reactions. DNA ranging from 70 bp to 10 kb is purified using a simple and fast bind-wash-elute procedure and an elution volume of 30–50 μl. An integrated pH indicator allows easy determination of the optimal pH for DNA binding to the spin column. The procedure can be fully automated on the QIAcube Connect. For optimal results it is recommended to use this product together with QIAvac 24 Plus. Performance The QIAquick Gel Extraction Kit enables removal of nucleotides, enzymes, salts, agarose, ethidium bromide, and other impurities from samples, ensuring up to 80% recovery of DNA (see figure " High recoveries from gels"). Using a microcentrifuge or vacuum manifold, DNA ranging from 70 bp to 10 kb is purified from 1–24 samples. Purified DNA can be used, for example, in sequencing (see figure " Reliable sequencing after gel extraction"). DNA fragments smaller than 70 bp or larger than 10 kb should be extracted with the QIAEX II Gel Extraction System.