Enzyme reagent kit QIAquick

for DNA extractionfor DNA cleanupfor biological samples

The QIAquick Gel Extraction Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease. ✓ 24/7 automatic processing of online orders ✓ Knowledgeable and professional Product & Technical Support ✓ Fast and reliable (re)-ordering Features Up to 95% recovery of ready-to-use DNA Fast and convenient procedure Cleanup of DNA up to 10 kb in three easy steps Gel loading dye for convenient sample analysis Combined kit for gel extraction and PCR cleanup Product Details The QIAquick Gel Extraction Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments from gels (up to 400 mg slices) or enzymatic reactions. DNA ranging from 70 bp to 10 kb is purified using a simple and fast bind-wash-elute procedure and an elution volume of 30–50 µl. An integrated pH indicator allows easy determination of the optimal pH for DNA binding to the spin column. The QIAquick PCR & Gel Cleanup Kit also provides buffers for purification of PCR products >100 bp and DNA of up to 10 kb. The procedure can be fully automated on the QIAcube Connect. Performance The QIAquick Gel Extraction Kit enables removal of nucleotides, enzymes, salts, agarose, ethidium bromide, and other impurities from samples, ensuring up to 80% recovery of DNA (see figure " High recoveries from gels"). Using a microcentrifuge or vacuum manifold, DNA ranging from 70 bp to 10 kb is purified from 1–24 samples. Purified DNA can be used, for example, in sequencing (see figure " Reliable sequencing after gel extraction").