For 50 preps: QIAamp Mini Columns, Tube Extenders (20 ml), QIAGEN Proteinase K, Carrier RNA, Buffers, VacConnectors, and Collection Tubes (1.5 ml and 2 ml)
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Features
Concentration of nucleic acids, with high input and low elution volumes
Efficient recovery of fragmented DNA and RNA
No organic extraction or ethanol precipitation
Removal of contaminants and inhibitors
Product Details
The QIAamp Circulating Nucleic Acid Kit greatly simplifies concentration and purification of free-circulating DNA and RNA from plasma or serum.
The kit can be automated on the QIAcube Connect.
Performance
Analysis of tumor-specific extracellular DNA fragments and mRNAs in the blood can enable specific detection of tumor types from a simple blood sample. These circulating nucleic acids are present in serum or plasma usually as short fragments, <1000 bp (DNA) or <1000 nt (RNA). In addition, miRNAs, as small as 21 nt, have the potential to provide biomarkers for certain cancers and disease states.
Principle
The QIAamp Circulating Nucleic Acid Kit simplifies isolation of circulating DNA and RNA from plasma or serum. No phenol–chloroform extraction is required. Nucleic acids bind specifically to the QIAamp Mini column, while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in 3 wash steps, leaving pure nucleic acids to be eluted in a buffer provided with the kit. The QIAamp Circulating Nucleic Acid technology yields circulating DNA and RNA from human plasma, serum, or urine.