Solution reagent kit qScript XLT SuperMix

reverse transcriptasefor RT-PCRfor cDNA synthesis

Premium Performance with SuperMix Benefits Features & Benefits Stabilized 5X concentrated master mix simplifies reaction assembly and improves accuracy by minimizing pipetting steps Optimized, double pre-primed SuperMix provides equal representation of 5′ and 3′ RNA sequences ≤ 1kb for quantitative and conventional two-step RT-PCR Improved RNA template binding affinity for increased yields with problematic sequences or complex secondary structure Perform more qPCR assays from limiting amounts of RNA with 2-3 fold improved cDNA yields over older enzyme technologies Broad linear dynamic detection range spanning up to 108 fold qScript XLT cDNA SuperMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease. Description qScript XLT cDNA SuperMix is a next-generation tool for first-strand cDNA synthesis, providing a highly sensitive and easy-to-use solution for two-step RT-PCR and RT-qPCR. qScript XLT is an engineered M-MLV reverse transcriptase mutant with reduced RNase H activity and improved yield and stability at higher temperatures. Combined with a precise mixture of reaction components, this SuperMix enables superior results over a wide dynamic range of input RNA, with up to 8-fold higher sensitivity than our previous qScript cDNA SuperMix cDNA synthesis kits, which utilize an engineered RNase H(+) reverse transcriptase mutant. Contents Single-tube, 5X concentrated reagent containing: Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, dTTP Recombinant ribonuclease inhibitor protein (RIP)