Research test kit MicroVue YKL-40

densityserumtissue

The MicroVue YKL-40 is a test to measure YKL-40, also known as human cartilage 39 (HC gp-39), in serum and human cell cultures. Though the role of YKL-40 is unknown at present, its pattern of expression and observed associations with various disease activities suggest it plays a role in tissue remodeling. YKL-40 — also known as human cartilage glycoprotein 39 (HC gp-39) is a 40 KD glycoprotein first described in whey secretions of nonlactating cows. Subsequently, its production by chondrocytes, synovial cells, activated macrophages, neutrophils, and osteosarcoma cells (MG-63) has been reported. It has both heparin and chitin binding domains and appears to be an autoantigen in rheumatoid arthritis. Early studies suggest YKL-40 is useful for researching: Rheumatoid Arthritis Early polyarthritis Osteoarthritis Giant cell arteritis Liver fibrosis Breast cancer Colorectal cancer Product Specifications Tests/kit 96-well/plate Performance Characteristics LOD*: 5.4 ng/mL LLOQ*: 15.6 ng/mL HLOQ*: 300 ng/mL Precision CVs within-run: 5.6%-6.6% Precision CVs between-run: 6%-7% Incubation time: 3 hours Cross-reactivity: YKL-40/HC gp-39 100% *LOD = Limit of Detection, LLOQ = Lower Limit of Quantification, HLOQ = Higher Limit of Quantification About the Assay Format 96-well microplate with reagents sufficient to test 40 samples in duplicate Species Reactivity Baboon, Rhesus and Cynomolgus Macaque, and Human Incubate 60 mintues at room temperature Wash 4 times with 1X Wash Buffer Wash 4 times with 1X Wash Buffer Add 100 µL Working Substrate Solution Read Optical Density at 405 nm Data Reduction: Linear Curve Fit