The Lyra Direct HSV 1+2/VZV Assay is a multiplex in vitro test that detects and differentiates Herpes simplex virus type 1, Herpes simplex virus type 2, and Varicella-zoster virus nucleic acids isolated and purified from cutaneous or mucocutaneous swab specimens obtained from symptomatic patients.
A multiplex real-time PCR reaction is carried out under optimized conditions in a single tube generating amplicons for HSV-1, HSV-2, VZV, and the Process Control (PRC). Identification of HSV-1, HSV-2, VZV, and the PRC occurs by the use of target-specific primers and fluorescent-labeled probes that hybridize to conserved regions in the genomes of HSV-1, HSV-2, VZV and the PRC.
The Lyra Direct HSV 1+2/VZV Assay has one-step reagent set-up and a three-step sample prep. Test results are available in less than 70 minutes. The Lyra Direct HSV 1+2/VZV Assay is available for use on Applied Biosystems® 7500 Fast Dx, QuantStudio™ Dx Real-Time PCR Instrument, and Cepheid® SmartCycler® II.
The Lyra Direct HSV 1+2/VZV Assay is one in a growing menu of PCR tests and is used as an aid in the differential diagnosis of Herpes simplex virus 1 and 2 (HSV-1 and HSV-2) and Varicella-zoster virus (VZV) also known as Human herpes virus 3. VZV is the causative agent for chicken pox and shingles.
One-step reagent set-up
Rehydration solution is simply added to the lyophilized Master Mix
Speed
Results in less than 60 minutes
Easy training
Simplified and uniform workflow with standard pipetting volumes
Refrigerated storage
No freezer needed. 2°C to 8°C storage
Long shelf life
2 years from date of manufacture
Room temperature set up
No ice or cooling block required