Clostridium difficile test kit PG0835
for hospital-acquired infectionsstoolfor real-time PCR

Clostridium difficile test kit
Clostridium difficile test kit
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Characteristics

Applications
for hospital-acquired infections
Micro-organism
Clostridium difficile
Sample type
stool
Analysis mode
for real-time PCR

Description

For in vitro diagnostic use. RIDA®GENE Clostridium difficile is a multiplex real-time PCR for the direct, qualitative detection of Clostridium difficile (16s-rDNA) and Clostridium difficile toxin A (tcdA) / B (tcdB) genes from human stool samples and cultures. The RIDA®GENE Clostridium difficile multiplex real-time PCR can be used as an aid in diagnosis of Clostridium difficile associated diarrhea (CDAD). General information: Clostridium difficile, a gram-positive, spore-forming anaerobic bacterium was first described in 1935 by Hall and O’Toole as a component of the intestinal microflora in healthy neonates. In the late 1970s, however, Clostridium difficile was identified as the cause of antibiotica-associated diarrhea and pseudomembranous colitis. Today Clostridium difficile is one of the most common causes of nosocomial diarrhea. Clostridium difficile is responsible for 15 – 25 % of antibiotica-associated diarrhea and nearly all cases of pseudomembranous colitis. The predisposing risk factors for CDAD are for example antibiotica exposure, advanced age as well as number and duration of hospitalization. However, Clostridium difficile infection is also seen in an increasing number of non-antibiotic-treated and non-hospitalized individuals. The symptoms range from mild diarrhea to intestinal infections of variable severity, including pseudomembranous colitis, the most severe form of antibiotic-induced inflammatory bowel disease. Clinically symptomatic cases are caused by toxigenic Clostridium difficile strains that produce toxin A and toxin B. In recent years the incidence and severity of Clostridium difficile infections increased worldwide.

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