CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Formulation - Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution -
It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 100 μg/mL.
Shipping - The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
N-terminal Sequence
Analysis - Ile
Structure / Form - Dimer
Predicted Molecular Mass - 26.6 kDa
SDS-PAGE - 30-35 kDa, reducing conditions
Endotoxin Level - <0.10 EU per 1 μg of the protein by the LAL method.
Activity - Bioassay data are not available.
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse,