Solution reagent 11415-EN

bovine serum albuminfor researchtissue

Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit . The specific activity is >12,500 pmol/min/μg, as measured under the described conditions. N-terminal Sequence Analysis - Trp27 Predicted Molecular Mass - 85 kDa SDS-PAGE - 84-93 kDa under reducing conditions. Carrier Free What does CF mean? CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA. What formulation is right for me? In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere. Assay Procedure Materials Assay Buffer: 25 mM Tris, 5 mM MgCl2, pH 7.5 Recombinant Human 5'‑Nucleotidase/CD73 Fc Chimera (rhCD73/mFc) Substrate: Adenosine monophosphate (AMP), 5 mM stock in deionized water Malachite Green Phosphate Detection Kit 96-well Clear Plate Plate Reader with Absorbance Read Capability Prepare a standard curve from the 1 M Phosphate Standard by adding 10 µL of the 1 M Phosphate Standard to 990 µL of Assay Buffer for a 10 mM stock. Continue by adding 10 µL of the 10 mM Phosphate stock to 990 µL of Assay Buffer for a 100 µM stock (this is the first dilution to use as a standard).