Stop Solution contains 15 vials (6 mL/vial) of 2N sulfuric acid for the stopping the action of horseradish peroxidase on the tetramethylbenzidine substrate in an ELISA.
PROCEDURE
Add 50-100 µL/well to stop the action of horseradish peroxidase on the substrate, tetramethylbenzidine. If the color in the wells is green or the color change does not appear to be uniform, gently tap the plate to ensure thorough mixing.
TECHNICAL HINTS & LIMITATIONS OF THE PROCEDURE
Caution: Wear protective gloves, clothing, and eye and face protection. Wash hands thoroughly after handling. Please refer to the MSDS on our website prior to use.
Specifications
Shipping Conditions - The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.