Protease reagent kit ARY025

antibodytissuechemiluminescence

Contains 4 membranes-2 spotted in duplicate with antibodies against 35 different proteases and 2 spotted in duplicate with antibodies against 32 different protease inhibitors General Assay Principle Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernates, cell and tissue lysates, serum, plasma, urine, saliva, or human milk samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any protein/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of protein bound. Chemiluminescence is detected in the same manner as a Western blot. Kit Contents 4 Array Membranes (2 of Proteases and 2 Protease Inhibitors) 4-Well Multi-dish Array Buffers Wash Buffer Detection Antibody Cocktail, Human Protease Array Detection Antibody Cocktail, Human Protease Inhibitor Array Streptavidin-HRP Chemiluminescent Detection Reagents Transparency Overlay Template Detailed Protocol Specifications Shipping Conditions - The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.