Research reagent 11349-IL series

solutioncytokinebovine serum albumin

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA. What formulation is right for me? In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere. Formulation - Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. Reconstitution - Reconstitute 10 μg size at 100 μg/mL in PBS. Reconstitute all the other sizes at 500 μg/mL in PBS. Shipping - The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. Endotoxin Level - <0.10 EU per 1 μg of the protein by the LAL method. Activity - Measured by its ability to induce IL-17 secretion by mouse splenocytes. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910. The ED50 for this effect is 0.100-1.00 ng/mL. Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1-5). The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. H