Solution reagent kit DY999B

for researchfor ELISA

Substrate Reagent Pack contains 16 vials of TMB ELISA Substrate producing color development when contacting HRP-conjugated or biotinylated, streptavidin-HRP bound detection antibodies. REAGENT PREPARATION Warm to room temperature before use. Protect from light. Add 100 µL of the mixture to each well and incubate at room temperature for 20 minutes while protecting from light. Add Stop Solution (Catalog # DY994) and read the microplate.