Enzyme reagent 9767-AR

bovine serum albuminfor researchtissue

Endotoxin Level - 60,000 pmol/min/μg, as measured under the described conditions. Source - E. coli-derived human Arginase 2/ARG2 protein Val23-Ile354 with an N-terminal Met and 6-His tag N-terminal Sequence Analysis - Met Predicted Molecular Mass - 37 kDa SDS-PAGE - 40 kDa, reducing conditions Carrier Free What does CF mean? CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA. What formulation is right for me? In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere. Assay Procedure Materials Assay Diluent: Deionized Water Recombinant Human Arginase 2/ARG2 (rhARG2) Urea, 100 mM stock in deionized water Substrate Buffer: 125 mM L-Arginine, 625 mM Glycine, pH 10.5 Manganese Chloride, 1 M stock in deionized water O-Phthaldialdehyde (oPA), (Sigma, Catalog # P0657), 50 mg/mL (373 mM) stock in DMSO N-(1-Naphthyl)ethylene-diamine dihydrochloride (NED) (Sigma, Catalog # N9125), 500 mM stock in DMSO 50 mM Boric Acid, 1 M Sulfuric Acid, 0.03% Brij-35 (w/v) [Caution: highly acidic, neutralize before disposal] 96-well Clear Plate