Protease reagent kit ARY021B

antibodytissuechemiluminescence

To simultaneously detect the relative changes of 35 proteases in a single sample. No specialized equipment is necessary Troubleshooting Guide General Assay Principle Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernates, cell lysates, serum, plasma, urine, saliva, or tissue lysate samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any protein/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of protein bound. Chemiluminescence is detected in the same manner as a Western blot. Kit Contents 4 Array Membranes 4-Well Multi-dish Array Buffers Wash Buffer Detection Antibody Cocktail Streptavidin-HRP Chemiluminescent Detection Reagents Transparency Overlay Template Detailed Protocol Specifications Shipping Conditions - The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.