Growth factor reagent CCM029

for research

Media for the low-serum, VEGF-free expansion of primary endothelial cells. Key Benefits Unique and optimized formulation that promotes endothelial cell growth. Free of Vascular Endothelial Growth Factor (VEGF) Includes a single supplement featuring key growth factors and small molecules 250 mL bottle results in less wasted media Why Use VEGF-free, low-serum Media to Grow Endothelial Cells? Primary endothelial cells are commonly used for the study of developmental and disease mechanisms involving the blood vessels, such as angiogenesis. Endothelial cells are phenotypically characterized as CD31+, KDR+, and vWF+ cells. Inclusion of Vascular Endothelial Growth Factor (VEGF) in endothelial cell media promotes cell proliferation. Under certain experimental conditions, a VEGF-free endothelial media may be beneficial. R&D Systems® Endothelial Cell Growth Media without VEGF is formulated to support the expansion of human primary endothelial cells under low-serum, VEGF-free conditions. This media has been tested for its ability to support CD31+, KDR+, and vWF+ human umbilical vein endothelial cells growth in vitro. Kit Contents This kit contains the following reagents to maintain and expand human and mouse endothelial cells in culture: Containing optimized concentrations of: Endothelial Cell Growth Base Media (250 mL) Endothelial Cell Growth Supplement without VEGF (50X) Human FGF basic Human LR3 IGF-1 Human EGF Heparin Hydrocortisone L-Ascorbic Acid Fetal Bovine Serum Note: The components for this kit require different storage/shipping temperatures and will arrive in separate packaging.