Real-time PCR system DropX-2000

digitalfluorescencewith touchscreen

The principle of Digital PCR is to divide nucleic acids template into separate reaction chambers, allowing for individual molecule quantification through PCR amplification and fluorescence signal detection. Unlike real-time PCR, digital PCR does not rely on Ct values or standard calibration curves. It offers exceptionally high sensitivity, precision, and repeatability, making it an absolute quantitative analysis technology. The DropX-2000 Droplet Digital PCR System puts this powerful technology at your disposal, enabling groundbreaking research at previously unattainable levels. The core principle of digital PCR is partitioning and Poisson distribution. DNA or RNA amplicons are divided into more than 20,000 droplets, with each droplet containing either 0 or 1 copy of the amplicon. After the completion of PCR thermal cycling, the droplets containing amplicons exhibit fluorescence, while those without copies do not. The number of fluorescent droplets is then counted as "0" or "1". By employing Poisson distribution, the concentration (copy number) of the sample can be calculated based on the number of fluorescent droplets. The DropX-2000 System utilizes advanced microfluidics technology to achieve sample partitioning, generating approximately 20,000 nanoliter-sized droplets per sample. The DropX-2000 system is well-suited for various applications, including tumor biomarker research, copy number variation analysis, viral pathogen detection, microbial detection, genetically modified organism detection, mRNA and miRNA detection, rare nucleic acid sample detection, NGS reference standard quality control, and gene editing result verification.