MagCore® Genomic DNA FFPE One-Step Kit is designed for purification of total DNA from FFPE tissues by using MagCore® instruments. It features the method, One-Step Heating, to melt paraffin and lyse tissue samples at the same time without harmful reagents involved such as xylene. Two protocols are designed and optimized for different sizes of tissues: 2 hrs for small samples/ 16 hrs for large samples. DNA will be extracted fast and economically based on the cellulose coated magnetic bead technology.
For tissue and forensic samples (up to 40mg)
Contents : pre-filled cartridges, Thermostable cap, proteinase K, PK storage buffer, Pipet tip & holder sets, Sula Oil, elution tubes
Shelf life : 18 months
Features
A full package for DNA purified from FFPE samples. It starts from the step of neutralizing paraffin to the final DNA extraction.
SULA oil (deparaffinization solution): no xylene involved
Thermostable cap is specially designed to cover the reaction well for an optimal condition
where lysis buffer and the FFPE sample can mix properly during the long processing period.
Applications
MagCore® Genomic DNA Tissue Kit is specific for the following samples:
Swab
Animal tissue
Stool
Feed-soil sample
Forensic sample
Performance
2-hr heating protocol works out
There are 2-hour heating and 16-hour heating protocols. It is suggested for a better performance that the 2-hour heating program is for small tissues and the 16-hour program is for large tissues.
Genomic DNA with high performance
The DNA extracted by MagCore® Genomic DNA FFPE One-Step Kit is high yield and suitable for downstream PCR and real-time PCR.