Research detection kit AlphaLISA®

apoptosisoncologyfor growth factors

The AlphaLISA® Human TGF-β1 Detection Kit is designed for detection and quantitation of human TGF-β1 (hTGF-β1) in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows negligible cross-reactivity with human hTGF-β2 and hTGF-β3. Formats: Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, Features: No-wash steps, no separation steps ELISA alternative technology Sensitive detection Broad sample compatibility Small sample volume Half the time of an ELISA assay Transforming growth factor beta 1 or TGF-β1, part of the TGF β cytokine superfamily, is a 25 kD disulfide-linked homodimer. TGF-β1 is produced by many cell types including immune cells and controls cell growth, proliferation, differentiation, and apoptosis by modulating many other cytokines and cytokine receptors. Serum and urine concentrations of TGF β1 are a useful marker for determining the status of patients with diabetic nephropathy in type II. AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity.