Research rapid test PG-Seq™

geneticgenomic DNAcell

The PG-Seq™ Rapid kit v2 has been developed to analyze picogram quantities of DNA (single/multi-cells or low template DNA) from an embryo biopsy for preimplantation genetic testing. The PG-Seq™ Rapid kit v2 utilizes whole genome amplification (WGA) and next generation sequencing (NGS) technology to accurately screen all 24 chromosomes for whole chromosome aneuploidy and sub-chromosomal abnormalities. Overiew Fast PGT-A prep: 3 hours from cell lysis to NGS ready Updated, streamlined, simple PGT protocol From DNA to data, the PG-Seq™ Rapid kit v2 includes all reagents required for cell lysis, whole genome amplification, indexing along with the PG-Find™ analysis software for automatic calling of aneuploidy and copy number variants. Accurate Copy Number Detection Extensively tested using over 100 cell lines and genomic DNA samples with known ploidy. Tested from whole chromosome aneuploidies down to segmental aberrations 7Mb in size with 30pg of genomic DNA or 5 cell fibroblast samples, representative of a trophectoderm biopsy. See PG-Seq™ Rapid kit v2 app note for more information. Flexible Kit Format The PG-Seq™ Rapid kit v2 has 48 rxns Two sets of 48 unique indexes are available, allowing up to 96 sample multiplexing (or up to 384 sample multiplexing available by custom request). Larger 40 µL WGA PCR 1 reaction volume enable options for alternative downstream processing. High WGA PCR 1 yield of 2-4 µg total DNA with only 23 cycles of PCR. Improved whole genome coverage and accuracy Enhanced PG-Find quality scores compared to previous versions of the kit signifies less noise, less bias, and higher confidence.