Fully automated sample preparation system AVENIO Edge

for next-generation sequencingfor sequencingNGS

Overview
Simplify your NGS library prep with the AVENIO Edge System, a fully automated IVD liquid handler for end-to-end library preparation, target enrichment, quantification, normalization, and pooling. The AVENIO Edge System is an all-in-one, pre-analytical platform that minimizes manual touchpoints, includes ready-to-load reagents, validated protocols, on‑board QC capabilities, and intuitive software to manage NGS sample workflows. Part of the Roche NGS Automation solution, the AVENIO Edge automates key steps to optimize lab resources and enable reliable walkaway operation.

Key product features

• Fully integrated on-deck thermal cycler for sample amplification
• Proprietary advanced bead-processing station for high sensitivity and yield
• Tip park to reduce reloading time, risk of contamination, and need for disposables
• AVENIO Designer Software: coding-free protocol creation and flexible configuration of user-defined workflows
• Fully barcoded reagents and consumables automatically loaded and tracked
• Integrated automated quantification (fluorescence-based) to minimize manual tasks and variability
• 2D imager for scanning 2D and 1D barcodes for sample and reagent traceability
• On-board pre-run inventory checks and pre-analytical system checks to help prevent errors

Benefits at a glance

• Minimal manual touchpoints streamlines daily routines
• Optimization of laboratory resources to maximise productivity
• Accurate, reliable, and reproducible results with reduced cross-contamination risk
• Set up to walk away in ~15–20 minutes; supports extended walkaway automation (up to ~2 days)
• Process examples: process 24 samples end-to-end in ~36 hours; run up to 4 panels in one run

Operational workflow highlights

• Create user-defined workflows with templated design software accessible remotely
• Load instrument with barcoded samples, reagents and consumables; automatic checks before run start
• Walk away and return later to collect normalized, pooled, sequencing-ready libraries
• Reagents come pre-kitted, barcoded, ready-to-use for simplified setup

Reagent & sample information

• Reagent design: Pre-filled, barcoded, ready-to-use; reagent kits optimized for multiple runs
• Sample type: Extracted DNA samples (support for plasma, cell lines, whole blood inputs noted)
• Workflows supported: Library preparation + hybridization-based target enrichment; multiple validated Roche protocols and a growing menu of user-defined workflows

Quality, traceability & compliance

• On-deck fluorescence-based quantification for QC
• Integrated process monitoring, audit trail and guidance (21 CFR part 11 subsection B compliance for traceability)
• Regulatory labels: US‑IVD and CE‑IVD (instrument intended for downstream diagnostic applications); reagents/kits described as Research Use Only where specified

Software & connectivity

• AVENIO Designer software for protocol design and user-defined workflows
• Traceability and audit features, process monitoring and user guidance
• Data export formats: CSV, XML, PDF (run exports); sample input accepted as .csv
• Interfaces: 2 external USB ports and 1 network port
• Connectivity: HL7 LIS connection and Roche remote service solution supported

Technical specifications

• Configuration: Floor model
• Throughput per run: 1 - 48 samples for library prep and target enrichment
• Runtime: Up to 31 hrs (including ~16 hrs hybridization time)
• Setup time: Approximately 15 minutes prior to the run
• Inventory check: Pre-run consumable list; pre-run check for empty positions and incorrectly placed items
• Quality control on deck: Fluorescence-based quantification
• Barcodes: 2D imager scans for 2D and 1D barcodes
• Regulatory label: US‑IVD, CE‑IVD
• Reagent design: Pre-filled, barcoded, ready-to-use; reagent kits optimized for multiple runs
• Sample types supported: Extracted DNA (including plasma, cell lines, whole blood)
• Workflows / maximum samples (per run) & estimated setup time: AVENIO Edge HyperPrep or AVENIO HyperPlus DNA library preparation — Maximum 48 samples — Setup ~20 minutes; AVENIO Edge Target Enrichment with KAPA HyperExome or custom panels — Maximum 48 samples — Setup ~20 minutes; AVENIO Edge HyperCap DNA library preparation and target enrichment — Maximum 24 samples — Setup ~20 minutes; Input control quantification/normalization/buffer exchange — Maximum 96 samples — Setup ~15 minutes; Post-capture pooling and quantification — Maximum 48 samples — Setup ~15 minutes
• Traceability & audit: 21 CFR part 11 compliant (subsection B), audit trail and process monitoring
• Data export: CSV, XML, PDF
• Interfaces: 2 external USB ports, 1 network port
• Connectivity: HL7 LIS connectivity, Roche remote service