Medium reagent KAPA HyperCap

buffer solutionmagnetic bead-basedfor oncology

Overview The KAPA HyperCap fixed (catalog) panels and companion kits provide off-the-shelf solutions for hybridization-based targeted enrichment in research workflows (For Research Use Only, RUO). They combine probe design expertise and controlled probe manufacturing to deliver consistent lot quality and high sequencing efficiency across oncology and hereditary-disease applications.

KAPA HyperCap Oncology Panel The Oncology panel is a ~214 Kb capture target covering the full coding sequences of 13 somatic oncology genes (ATM, BRCA1, BRCA2, BRIP1, CHEK2, EGFR, ERBB2, KRAS, MET, MYCN, RAD51C, RAD51D, TP53) plus hotspot variants across ~69 additional genes. It is optimized for challenging input types (FFPET/FFPE and cfDNA) with tuning for high uniformity and specificity. Reported performance examples include >94% error-free positions from low-quantity/low-quality FFPET DNA and strong deep-coverage metrics (uniformity: >92% bases within 0.5x–2x median; >88% exon bases covered >300x).

KAPA HyperCap Heredity Panel The Heredity panel is an approximately 10 Mb capture target covering 3,332 genes associated with hereditary-disease research and includes ClinVar pathogenic/likely pathogenic variants. It is engineered for sequencing efficiency with optimized uniformity and low duplication rates, delivering high percent target coverage (example: 98.5% of target bases covered at 30x; mean coverage ~92.6x). Lot-to-lot probe QC supports consistent performance.

Benefits at a glance

• High sequencing quality and efficiency for FFPET and cfDNA with optimized probe design and manufacturing.
• Automation-friendly KAPA HyperCap workflows compatible with KAPA HyperPrep, KAPA HyperPlus and KAPA Universal UMI Adapters; supports pre-capture multiplexing to increase throughput.
• Targeted gene content: oncology panel for key somatic genes and hotspots; heredity panel for thousands of hereditary-disease genes and ClinVar content for germline research.
• Off-the-shelf availability as catalog panels and kits with per-lot NGS probe QC.

Selected example catalog items (material number — product description — EAN) KAPA HyperCapture Reagent Kit, 24 rxn — 07613336198186
KAPA HyperCapture Reagent Kit, 96 rxn — 07613336198179
KAPA HyperCapture Reagent V2 Kit, 24 rxn — 07613336242315
KAPA HyperCapture Reagent V2 Kit, 96 rxn — 07613336242322
KAPA HyperCap Heredity panel, 24 rxn — 07613336188408
KAPA HyperCap Heredity panel, 48 rxn — 07613336188415
KAPA HyperCap Heredity panel, 96 rxn — 07613336188422
KAPA HyperCap Heredity panel, 192 rxn — 07613336188439
KAPA HyperCap Oncology panel, 12 rxn — 07613336188361
KAPA HyperCap Oncology panel, 24 rxn — 07613336188378
KAPA HyperCap Oncology panel, 48 rxn — 07613336188385
KAPA HyperCapture Bead Kit, 24 rxn — 07613336198209
KAPA HyperCapture Bead Kit, 96 rxn — 07613336198193
KAPA Universal Enhancing Oligos, 24 rxn — 07613336198162
KAPA Universal Enhancing Oligos, 96 rxn — 07613336198155

Performance & workflow notes Validation summaries include performance using FFPET DNA workflows (KAPA HyperCap FFPET DNA Workflow), cfDNA workflows (KAPA HyperCap cfDNA Workflow), and both single-sample and multiplexed capture approaches. Example validation inputs: 10 ng FFPE DNA, 10 ng cfDNA, 100 ng gDNA for pre-capture multiplexing. Reported metrics include on-target percentage, fold-80 base penalty, PCR duplication rates, percent bases at specified depths (e.g., >=30x), and median read counts after UMI deduplication for reference samples.

Technical specifications

• Regulatory use: For Research Use Only (RUO). Not for diagnostic use.
• Product family: KAPA HyperCap catalog panels and companion kits (reagents, bead kits, resuspension buffers, enhancing oligos, etc.).
• Panel target sizes: Oncology ~214 Kb (13 full CDS genes + hotspot content); Heredity ~10 Mb (3,332 genes + ClinVar content).
• Sample compatibility: FFPET/FFPE tissue, circulating cell-free DNA (cfDNA), genomic DNA (gDNA).
• Typical validated inputs: 10 ng FFPE DNA; 10 ng cfDNA; 100 ng gDNA for multiplexed pre-capture workflows.
• Key reported metrics: Oncology example — >94% error-free positions; >92% bases within 0.5x–2x median; >88% exon bases >300x. Heredity example — 80.9% reads on-target; 1.42 fold-80 base penalty; 2.2% PCR duplication; 98.5% target bases at 30x; mean coverage ~92.6x.
• Workflow compatibility: KAPA HyperCap workflows including KAPA HyperPrep, KAPA HyperPlus, KAPA EvoPrep Boost / EvoPlus Boost; supports UMI adapters and single-library or multiplexed pre-capture strategies.
• Quality control: NGS probe QC performed on every lot to ensure consistency.
• Availability: Off-the-shelf catalog panels and kits; multiple pack sizes/reaction counts (examples: 12, 24, 48, 96, 192, 384 reactions depending on item).