Solution reagent kit KAPA UDI

for researchliquid

For Research Use Only. Not for use in diagnostic procedures. Overview Full-length adapters are compatible with ligation-based library construction workflows for direct and targeted multiplexed DNA and RNA (cDNA) sequencing applications on the Illumina® platform. Since sequencing barcodes are added during an adapter ligation step, full-length adapters are particularly well suited for PCR-free workflows. KAPA Unique Dual-Indexed (UDI) Adapters have a standard Illumina adapter backbone. They are therefore easily incorporated in new and existing Illumina sequencing workflows, and do not require customized sequencing or indexing primers. A combination of 8-nt sequencing barcodes on each of the i5 and i7 adapter oligos supports paired-end sequencing on 1- , 2- and 4-channel Illumina instruments. KAPA UDI Adapters support a broad range of sample pooling (2- plex up to 96-plex, as appropriate for your workflow), for target enrichment and/or sequencing. Unique dual-indexing is recommended for all applications and Illumina sequencers. Product highlights Fewer misassigned reads improve confidence in results Index misassignment during multiplexed sequencing may be the result of index hopping, barcode or sample cross-contamination, template switching during PCR amplification of pooled samples, and/or sequencing/analysis errors; some of which may be mitigated by adapter design and quality Unique dual-index combinations in KAPA UDI Adapters allow reads with unexpected barcode combinations to be filtered out prior to data analysis