Solution reagent kit KAPA HyperPETE

sample preparationfor researchfor DNA extraction

As next-generation sequencing (NGS) sample preparation entails a multitude of steps and each step is critical for the overall sequencing success and efficiency, all steps need to be optimized in terms of reagents and protocol. Considering the precious nature of NGS samples, an integrated workflow with optimized reagents is necessary converting the samples into a sequencing-ready library. NGS sample types can differ in terms of their: Ease of sequencing - uniform or with secondary structures or GC-rich areas Quality - freshly extracted or preserved (formalin fixed paraffin embedded - FFPE) Flexible Workflows - DNA and RNA workflows optimized for applications Taking into account all these variations, it is important to use library preparation reagents that can work efficiently with all these sample types. High-quality library preparation reagents help in obtaining uniform and improved coverage as well as lower duplication rates. Depending on the end goal (for example, whole exome sequencing), a target enrichment step may also be required for enriching specific regions of interest. Use of high-efficiency target enrichment reagents helps in obtaining uniform and high depth of coverage with high specificity (on-target rate) over the targeted regions. The KAPA HyperPETE Workflow combines validated products for both KAPA Library Preparation and KAPA Target Enrichment. It provides all accessories and reagents needed for sample preparation, from DNA or RNA to the sequencer in a single-day workflow. The new KAPA HyperPETE Panels provide high capture efficiency, sequencing uniformity with less optimization and higher variant detection confidence.