ELISA kit AccuSignal™

for infectious diseasesfor researchEscherichia coli

This kit contains: E. coli-HCP antibody coated 96-well strip plate, Plate Sealer, E. coli-HCP standard, E. coli-HCP detection antibody, sample buffer, Wash Buffer, TMB Buffer, Stop Buffer, and protocol. Application Details Application Note: The AccuSignal™ E. coli HCP ELISA Kit is a sandwich, enzyme-linked immunosorbent assay. The impurities in process-derived samples containing E. coli proteins are captured and detected in a microtiter-type plate by using pre-coated, purified, rabbit polyclonal, E. coli-HCP-specific antibodies. The immunological reaction after the addition of TMB results in a chromogenic change that can be measured photometrically at 450 nm with reference wavelength at 630–650 nm. The O.D. in the wells is directly proportional to the amount of HCP concentration and correlates to the amount of HCP present in the original sample. The HCP concentration can be calculated based on the calibration curve generated with the provided E. coli-HCP standard. Shipping & Handling Shipping Condition: Wet Ice Storage Condition: The E. coli HCP Kit and reagents should be stored at 2-8°C. Allow reagents to reach room temperature (18-26°C) before use and may be used until the expiration date. It is recommended to aliquot the reconstituted standard solution and store it at -20°C to avoid freeze/thaw cycles. Expiration: See kit insert for complete instructions. The antibodies in the kit have been specifically designed to detect HCP contaminants and to measure analytes from crude cellular harvests to process intermediates. The E. coli ELISA Kit uses purified antibodies generated against a mixture of proteins of mock induced E. coli cell lysate.