Prepare FemtoMax™ Super Sensitive Chemiluminescent HRP Substrate for use in microwell or membrane applications by mixing 1 mL of Luminol Reagent (Reagent A) with 1 mL of Reaction Buffer (Reagent B). Mix well. Protect from light. Larger or smaller volumes of the substrate can be prepared by mixing components at the same 1:1 ratio. FemtoMax™ Super Sensitive Chemiluminescent HRP Substrate is a highly sensitive detection reagent. Always carefully optimize all components of individual assays (antigens, antibodies, conjugates…) to minimize background reactivity associated with non-specific binding.
Shipping & Handling
Shipping Condition: Wet Ice
Storage Condition:
Store container at 4° C prior to opening. Protect from moisture and light. No special shipping conditions or precautions are required.
Product Details
Description:
Chemiluminescent FemtoMax™ Super Sensitive HRP Substrate for Microwell and/or Membrane (2 component system) - FEMTOMAX-020
Synonyms:
Peroxidase Substrate, ECL HRP Substrate, Chemiluminescent Femtomax™ Super Sensitive horseradish peroxidase (HRP) Substrate For Microwell And/Or Membrane (2 Component System)
FemtoMax™ Super Sensitive Chemiluminescent HRP Substrate is an extremely sensitive, nonradioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP). FemtoMax™ is designed for both Western blotting and enzyme-linked immunosorbent assay (ELISA) use. FemtoMax™ easily allows for the detection of femtogram (10-15) amounts of antigen using photographic film or other imaging methods, including highly sensitive CCD cameras.