Monitor adherent cell morphology changes objectively to determine live/dead cell counts or classify cells based on morphology via label-free multivariate analysis.
Software Overview
The Incucyte® Advanced Label-Free Classification Analysis add-on for the Incucyte® Cell-by-Cell Analysis enables automated identification of cell morphology changes using label-free, integrated image acquisition, segmentation and multi-variate analysis of cell shape. Track biological changes using unbiased, smart defaults – opening up new possibilities in comparison of test results across experiments to:
Classify cells automatically and label-free based on morphology
Perform unbiased analysis of cell morphology
Conduct label-free phenotypic screening
Capturing data-rich information without the use of fluorescent labels has never been easier!
Incucyte® Advanced Label-Free Classification Analysis Workflow shows the steps required to run a label-free live/dead assay. Images are automatically acquired and segmented using the Incucyte® Cell-by-Cell Analysis Software Module. With the Incucyte® Advanced Label-Free Classification add-on, morphological features are obtained and a classifier is trained using live and dead control wells. This classifier is then applied to all wells and timepoints to achieve number and % live and dead cells per image.
Make informed decisions on morphological changes with ease analysis,
Utilize integrated multivariate analysis and smart default settings for classifying
morphological differences
Track adherent cell populations kinetically and easily visualize changes in morphology