Research test kit Octet®

immunoassayautomatedenzymatic

The Ready BLI Detection Kit - Residual Protein A (RPA) applies the principles of biolayer interferometry (BLI) to enable sensitive, accurate quantitation of leached Protein A in bioprocess samples while maintaining a simple workflow. The kit utilizes a validated sample treatment method for dissociating Protein A from antibodies that does not require boiling, neutralization, or centrifugation steps. Dip and Read RPA biosensors with pre-immobilized chicken anti-Protein A antibody are sequentially dipped into treated samples, buffers, and detection antibody arrayed in 96-well microplates to create a microscale immunoassay on the biosensor tip. Signal detection occurs in real time at the secondary antibody step without need for conjugates or enzymatic reactions. Quantitation of recombinant Protein A constructs and MabSelect SuRe™ Sensitivity down to 100 pg/mL Automated detection with minimal hands-on time