Buffer solution reagent kit G2002-100ML

for protein extraction

RIPA Lysis Buffer (RIPA Lysis Buffer) is a traditional rapid lyse solution for cells and tissues. Protein samples obtained from tissues and cells cleaved by RIPA lysate can be used for routine PAGE, Western, immunoprecipitation (IP), co-IP and other experiments.RIPA cracking fluid has many formulations, according to its cracking effect is mainly divided into strong, medium and weak three kinds. This product is a strong cracking liquid of RIPA. Its main components include 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1 mM EDTA-2Na, 1% Triton X-100, 1% sodium deoxycholic acid and 0.1% SDS.This product is suitable for animal or plant tissue and cell samples, can also be used for fungal or bacterial samples. Storage and Handling Conditions Wet ice bag transportation; Stored at 4℃ in the dark; valid for 18 months. Preparation for the experiment Protease inhibitor is provided. The RIPA lysate (strong) should be added with protease inhibitors before use. G2006, G2007, G2008, etc., are recommended to prevent protein degradation. The RIPA lysate (strong) mentioned in the following usage means that protease inhibitors have been added. Assay Protocol / Procedures