Puromycin reagent S250J0

for scientific researchtissueclinical

Puromycin is an aminonucleoside antibiotic produced by fermentation and metabolism of Streptomyces alboniger. The inhibition of protein synthesis kills gram-positive bacteria as well as various animal and insect cells, it is also applicable for E.Coli under certain circumstances. As a analogue to the 3’ terminal of Aminoacyl -tRNA molecule, puromycin can bind to the A site of ribosome and be added to the extended peptide chain. However, puromycin will no longer react after binding to the A site resulting in premature termination of protein synthesis and releases premature peptides from the C end containing puromycin. Resistance to puromycin is conferred by the puromycin N-acetyl-transferase gene (pac) from Streptomyces. Genetic engineering research uses pac gene derived from puromycin producing bacteria Streptomyces alboniger. Puromycin is commonly used for screening and maintaining the cultivation of stable transfection mammalian cells containing pac gene. The general application of puromycin in the selection of stable cell strains is related to the characteristics of lentivirus vectors, most commercial lentivirus vectors carrying pac genes nowadays. Under certain circumstances, puromycin can also be used to screen and transform E.Coli carrying pac gene. The product is prepared using water-for-injection. This product is only used for scientific research and production, tissue and cell culture in vitro. It is prohibited for clinical use.