Food safety test kit LSY-10007

for animalsdensitytissue

This test kit is based on theindirect competitive enzyme immunoassay for the detection of Chloramphenicol in the sample.The coupling antigenis pre-coated on the micro-well stripes. The Chloramphenicol in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Chloramphenicolantibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Chloramphenicol in it. This value is compared to the standard curve and the Chloramphenicol concentration is subsequently obtained. 2. Technical specifications Sensitivity: 15 ppt Incubation Temperature: 25℃ Detection limit: Tissue(method 1)7.5ppt Tissue(method 2) 15ppt Egg 15ppt Recovery rate Tissue, egg95±25% Cross-reaction rate: Chloramphenicol100% Thiamphenicol< 0.1% Florfeniol< 0.1% 4. Materials required but not provided 1) Equipments:microplate reader,homogenizer, nitrogen-drying device,vortex, centrifuge, measuring pipets, andbalance( asensibility reciprocalof0.01 g), incubator. 2) Micropipettors: single-channel 20-200 µL, 100-1000 µL, andmulti-channel 30～300 μl. 3) Reagents: Ethyl acetate,N-hexane, NaOH, K2HPO4·3H2O, HCl. 5. Sample pre-treatment Instructions Thefollowing pointsmust be dealt with beforethe pre-treatment ofany kind ofsample: 1) Only thedisposable tips can be used for the experiments and the tips must be changed when used forabsorbingdifferent reagents; 2) Before the experiment, eachexperimentalequipment must be checked to beclean and should be re-cleaned ifnecessary,in order to avoid thecontamination whichinterfereswith the experimental results.