Food safety test kit LSY-10005

for animalsdensitytissue

This test kit is based on the indirect competitive enzyme immunoassay for the detection of Neomycin in the sample.Thecoupling antigenis pre-coated on the micro-well stripes. TheNeomycin residues in the sampleand thecoupling antigen pre-coated on the micro-well stripes compete for the anti-Neomycinantibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with theNeomycin in it. This value is compared to the standard curve and theNeomycin concentration issubsequently obtained. 2. Technical specifications Sensitivity: 0.1ppb Incubation Temperature: 25℃ Detection limit Tissue, milk, milk powder, feed4ppb Duck8ppb Honey7ppb Egg3ppb Pig urine1ppb Recovery rate Tissue95±25% Chicken liver, Pig liver80±25% Milk,milk powder, honey, egg, feed, pig urine90±30% Cross-reaction rate Neomycin100% 4. Materials required but not provided 1) Equipment:microplate reader, printer,homogenizer,vortex, centrifuge, measuring pipets, andbalance( asensibility reciprocalof0.01g), incubator. 2) Micropipette: single-channel 20-200µL, 100-1000µL, andmulti-channel30～300µL. 3) Reagents: Trichloroacetic acid(TCA), NaOH, HCl, deionized water. Thefollowing pointsmust be dealt with beforethe pre-treatment ofany kind ofsample: 1)Only thedisposable tips can be used for the experiments and the tips must be changed when used forabsorbingdifferent reagents; 2)Before the experiment, eachexperimentalequipment must be checked to beclean and should be re-cleaned ifnecessary,in order to avoid thecontamination thatinterfereswith the experimental results.