Food safety test kit LSY-10060

for animalsdensitytissue

This test kit is based on the competitive enzyme immunoassay for the detection of Avermectin in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Avermectin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Avermectin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Avermectin in it. This value is compared to the standard curve and the Avermectin concentration is subsequently obtained. 2. Technical specifications Sensitivity: 0.5ppb Detection limit Tissue 4ppb Liquid milk 5ppb Note: ppb= ng/ml or ng/g Cross-reaction rate Avermectin 100% Ivermectin 25% Eprinomectin 10% Doramectin 6% Recovery rate 90±30% 3. Components 1） Micro-well strips: 12 strips with 8 removable wells each 2） 6× standard solution (1mL each): 0ppb,0.5ppb,1.5ppb, 4.5ppb,13.5ppb,40.5ppb 3） 11X Concentrated Enzyme conjugate (0.7 mL) 4） Enzyme conjugate dilution (7 mL) 5） Substrate A (7 mL) 6） Substrate B (7 mL) 7） Stop solution (7 mL) 8） 20×concentrated washing buffer (30 mL) 9） Redissolving solution (50 mL) 10） Tissue sample treatment solution (10mL) 4. Materials required but not provided 1） Equipments:microplate reader (450nm, 630nm), printer, homogenizer, nitrogen-drying device, vortex, shaker, centrifuge (4000g and above), measuring pipets, balance (a reciprocal sensibility of 0.01 g), incubator (25℃), timer; 2） Micropipettors: single-channel 20-200 µL, 100-1000 µL, and eight-channel 30～300 µl; 3） Reagents (AR): Methanol, deionized water