Infectious disease test kit LSY-30048

veterinaryfor pigletsfor animals

African swine fever (ASF) is a severe, acute, highly contagious infection of pigs caused by African swine fever virus (ASFV). ASF is characterized by high fever, cyanosis of the skin, severe bleeding of internal organs, respiratory disorders, neurological symptoms, short course of illness and high mortality. This kit based on VP72 antigen expression and specificity monoclonal antibody (McAb), adopting blocking enzyme-linked immunosorbent assay (b-ELISA), equipped with a full range of diagnostic reagents and materials, one box can detect 92 pieces of serum (or partial) according to the circumstance, can be used in African swine fever antibody detection, high sensitivity, strong specificity, repeatability, and the characteristics of the kit itself without infectivity. Blocking enzyme-linked immunosorbent assay(b- ELISA) for specific African swine fever virus antibodies detection in swine serum samples. 2. Principle The specific antibodies in the serum to be tested block the binding of ASFV and McAb. Add the serum to be tested into the 96 well enzyme plate hole coated with ASFV recombinant protein antigen. If there is specific antibody of ASFV in the serum to be tested, it will bind with the specific coated antigen. After blocking, add the binding reaction of enzyme-specific monoclonal antibody and antigen. The unbound enzyme-specific monoclonal antibody will be washed away and no color will appear after adding the substrate. On the contrary, if there is no specific antibody of ASFV in the serum to be tested, there will be color development after the substrate is added.