Brucellosis test kit LSY-30036

goatfor animalsfor antibodies

The Brucellosis antibody ELISA kit is used to test Brucellosis antibody in serum of bovine, goat, sheep, pig and dog etc. This kit use competitive ELISA method, Brucellosis antigen is pre-coated on enzyme micro-well strips. When testing, add diluted serum sample, after incubation, if there is Brucellosis specific antibody, it will combine with the pre-coated antigen, discard the uncombined antibody and other components with washing; then add enzyme labled anti-Brucellosis virus monoclonal antibody, antibody in sample block the combination of monoclonal antibody and pre-coated antigen; discard the uncombined enzyme conjugate with washing; Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample, use ELISA reader at 450nm wavelenth to measure the absorbance A value in reaction wells after adding stop solution to stop the reaction. 3. Material required not provided 1) Micropipette: 10ul-100ul, 100ul-1000ul. 2) Disposable pipette tips. 3) Graduate: 500ml. 4) Microplate Reader: 96 wells. 5) Distilled water or deionized water. 6) Microplate Washer 4. Sample preparation Take animal whole blood, get serum by using regular method, the serum should bright and no hemolysis 5. Washing buffer preparation Return 10X Concentrated washing buffer into room temperature before use, if there is salt crystals, shake to make it dissolved, then dilute it at 10 times with distilled water or deionized water. The diluted washing buffer can store at 4℃for about 1 week. 6. Notes 1) Return all reagents into room temperature before use, shake it evenly before use, and store back to 2-8℃after usage.