Infectious disease test kit LSY-30043

for animalsfor antibodiesvirus

Influenza virus, referred to as influenza virus, can be divided into three types: A, B, and C. Among them, influenza A virus is divided into many subtypes according to different hemagglutinin H antigen and neuraminidase N antigen. Influenza A viruses are currently known to infect a variety of animals including birds, humans,pigs, horses, dogs, whales etc. This kit use blocking ELISA method, to detect influenza A virus antibody in serum of multiple animal likeSwine, birds, dog, horse etc. When testing, add diluted serum sample, after incubation, if there is influenza A virus specific antibody, it will combine with the pre-coated antigen, discard the uncombined antibody and other components with washing; then add enzyme labled anti-Influenza A virus monoclonal antibody, antibody in sample block the combination of monoclonal antibody and pre-coated antigen; discard the uncombined enzyme conjugate with washing; Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample, use ELISA reader at 450nm wavelenth to measure the absorbance A value in reaction wells after adding stop solution to stop the reaction. 3.Material required not provided 1) Micropipette: 10ul-100ul, 100ul-1000ul. 2) Disposable pipette tips. 3) Graduate: 500ml. 4) Microplate Reader: 96 wells with 450/630nm wavelength. 5) Distilled water or deionized water. 6) Bottle washer or Microplate Washer 4. Sample preparation Take animal whole blood, get serum by using regular method, the serum should bright and no hemolysis .