This test strip is based on fast immuno-chromatography technique to detect foot-and-mouth disease type A antigen in bovine, goat and pig serum, plasma and whole blood qualitatively. When testing, FMD Ag in sample combine with antibody coated by colloid gold forming complexes, moving forward to the other head. When reaching the T-line, the specific antibody on the membrane capture the complexes and appear a T line. C line is Control line, C line appears, means the test is valid.
2. Specifications: 40 tests/kit
3.Components
1) FMD Type A antigen Test cassette 40 pieces
2) Dropper 40 pieces
3) Manual 1 piece
4. Sample preparation
4.1 Whole blood
Collect anticoagulated whole blood, use for that very day;
4.2 Serum
Collect blood, put at 37℃for 1-2h, take the supernatant, centrifuge at 1500r/min for 10min,separate the serum. The serum can store for 2-3 days at 4℃, for long term storage, store at -20℃in frozen.
1) Read the manual carefully and return the test card and sample to room temperature before use.
2) Take out test card, use dropper to absorb the sample, drop 2-3 drops into well mark “S”; or use Micropipette to transfer 70ul sample into well mark “S” .
6. Results
Negative(-): no color reaction on Test Line (T Line),only on Control Line(C Line) being seen red color reaction. It means there is no FMD type A Ag in sample or the levels is lower than the detection threshold;
Positive(+): Both on T Line and C line being seen red color reaction. It means there is FMD type A Ag in sample;
Invalid: No color reaction on C Line. It means the operation is wrong or the test card is invalid.