The Rotavirus antigen rapid test kit is based on colloidal gold immuno-chromatography technique to detect type A Rotavirus antigen in feces of cattle, pig and dog. After adding sample, the sample move along with the colloidal gold labeling Rotavirus antibody, if there is Rotavirus antigen in the sample, it combines with antibody on T line and show wine-red color; if there is no Rotavirus antigen, then no color reaction.
3. Test procedure
1) Use swab to collect fresh feces or take sample from Rectum directly. If it is for Rotavirus screening for animals without diarrhea, please take sample from Rectum. We suggest that do not take dry feces sample, because collecting dry feces sample will lead to collecting too much sample and appear false positive.
2) Insert the swab into the tube with sample buffer immediately, rotate the swab strongly on tube well for at least 10 times and mix the solution evenly, making the sample been dissolved in sample buffer fully. Press the swab on tube well above the liquid level, making the liquid been pressed out fully, discard swab.
3) If the feces particles is big, be static for 1-2min, make big particles precipitate.
4) Take out test card from sealed bag. Put it on flat surface, use dropper to draw upper-layer bright liquid, drop 5 drops slowly, one by one, to the sample hole with “S” mark.
4.Results judgment
1) Negative: only control line is seen wine red;
2) Positive: control line and test line are both seen wine red;
5. Note
1) Do not use product with broken Aluminum foil bag package. Use test card in 1 hour after taking out.