Food safety test kit LSY-10068

for animalsdensitysodium

This test kit is based on the indirect competitive enzyme immunoassay for the detection of Trenbolone in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Trenbolone in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Trenbolone antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Trenbolone in it. This value is compared to the standard curve and the Trenbolone concentration is subsequently obtained. 2. Technical specifications Sensitivity: 0.03ppb Incubation Temperature: 25℃ Detection limit: Tissue 1.5ppb Milk,urine 0.6ppb Milk powder 1.2ppb Recovery rate Tissue, milk, urine, milk powder 70%~120% Cross-reaction rate: Trenbolone 100% 4. Materials required but not provided 1) Equipments:microplate reader, homogenizer, vortex, centrifuge, measuring pipets, and balance( a sensibility reciprocal of 0.01 g), incubator, printer. 2) Micropipettors: single-channel 20-200 µL, 100-1000 µL, and multi-channel 30～300 μl. 3) Reagents: Methanol, Sodium hydroxide (NaCl). The following points must be dealt with before the pre-treatment of any kind of sample: 1) Only the disposable tips can be used for the experiments and the tips must be changed when used for absorbing different reagents; 2) Before the experiment, each experimental equipment must be checked to be clean and should be re-cleaned if necessary, in order to avoid the contamination which interferes with the experimental results.