Food safety test kit LSY-10046

for animalsfor antibodiesdensity

This test kit is based on the competitive enzyme immunoassay for the detection of MQCA in the sample. The coupling antigens are pre-coated on the micro-well stripes. The MQCA in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-MQCA antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the MQCA in it. This value is compared to the standard curve and the MQCA concentration is subsequently obtained. 2. Technical specifications Sensitivity:0.5 ppb Incubator temperature: 25℃ Incubator time: 30min～15min Detection limit: Tissue 1 ppb Cross-reaction rate: MQCA 100% Olaquindox <0.1% Recovery rate: Tissue 85%±10% 3. Components 1） Micro-well strips: 12 strips with 8 removable wells each 2） 6× standard solution (1 mL each): 0 ppb, 0.5 ppb, 1.5 ppb, 4.5 ppb, 13.5 ppb, 40.5 ppb 3） Concentrated Enzyme conjugate (1 mL) red cap 4） Antibody working solution (8 mL) blue cap 5） Substrate A solution (7 mL) white cap 6） Substrate B solution (7 mL) black cap 7） Stop solution (7 mL) yellow cap 8） 20× concentrated washing buffer (40 mL) white cap 9） Redissolving solution (50 mL) transparent cap Materials required but not provided 1) Equipments:microplate reader (450 nm / 630 nm), homogenizer, oscillator, centrifuge, measuring pipets, nitrogen-drying device and balance (a sensibility reciprocal of 0.01 g), Incubator. 2) Micropipettors: single-channel 20 to 200 µL and 100 to 1000 µL and multi-channel 30～300 µl; 3) Reagents:Ethyl acetate, n-hexane, HCl, NaCl, CH3CN