Food safety test kit LSY-10067

for animalsdensitytissue

This test kit is based on the indirect competitive enzyme immunoassay for the detection ofCefalexin insamples. The coupling antigensare pre-coated on the micro-well stripes.TheCefalexin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Cefalexin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with theCefalexin in the sample. This value is compared to the standard curve and theCefalexin concentration is subsequently obtained. 2. Technical specifications Sensitivity:0.3 ppb Incubation Temperature: 25℃ Detection limit: Tissue 10ppb Egg 15ppb Liquid milk 5ppb Milk powder 24ppb Cross-reaction rate: Cefalexin 100% Recovery rate Tissue100%±30% Egg, liquid milk, milk powder 90%±30% Materials required but not provided 1) Equipments:microplate reader, printer, homogenizer, nitrogen-drying device, vortex, centrifuge, measuring pipets, balance (a sensibility reciprocal of 0.01 g), incubator. 2) Micropipettors: single-channel 20-200 µL,100-1000 µL, and multi-channel 30～300 µl ; 3) Reagents: Methanol, NaOH 5. Sample pre-treatment 1) Only the disposable tips can be used for the experiments and the tips must be changed when used for absorbing different reagents; 2) Before the experiment, each experimental utensil must be clean and should be re-cleaned if necessary, in order to avoid the contamination that interferes with the experimental results. Solution preparation before sample pre-treatment: 1) 1M NaOH:Take 4.0g NaOH, add deionized waterto100mL, mix it evenly.