Food safety test kit LSY-10065

for animalsdensitytissue

This test kit is based on the indirect competitive enzyme immunoassay for the detection of Trimethoprim in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Trimethoprim in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Trimethoprim antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Trimethoprim in it. This value is compared to the standard curve and the Trimethoprim concentration is subsequently obtained. 2. Technical specifications Sensitivity: 0.5 ppb Incubation Temperature: 25℃ Detection limit: Tissue 5ppb Cross-reaction rate: Trimethoprim 100% Sulfamerazine ＜0.1% Sulfaquinoxaline <0.1% Sulfamethazine ＜0.1% Sulfamonomethoxine <0.1% Sulfamethoxazole <0.1% Sulfathiazole <0.1% Sulfamethoxypyridazine <0.1% Sulfaguanidine <0.1% Sulfadimethoxine <0.1% Sulfadiazine <0.1% Sulfameter <0.1% Recovery rate: Tissue 60%～120% 4. Materials required but not provided 1) Equipments: microplate reader, homogenizer, oscillator, centrifuge, balance (a sensibility reciprocal of 0.01 g), graduated pipette, incubator. 2) Micropipettors: single-channel 20~200 µL and 100~1000 µL, and multi-channel 30~300 µL; 3) Reagents: NaOH. The following points must be dealt with before the pre-treatment of any kind of sample: 1) Only the disposable tips can be used for the experiments and the tips must be changed when used for absorbing different reagents;