Food safety test kit LSY-10021

for animalsdensityurine

This test kit is based on the competitive enzyme immunoassay for the detection of Diazepam in the feed, urine, liver and meat. The coupling antigen is pre-coated on the micro-well stripes. The Diazepam in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Diazepam antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Diazepam in it. This value is compared to the standard curve and the Diazepam concentration is subsequently obtained. 2. Technical specifications Sensitivity: 0.1 ppb Detection limit Tissue 1 ppb Urine 1 ppb Feed 10 ppb Recovery rate Urine 80 ±10% Feed 75 ±10% Tissue(meat, liver) 85 ±10% Cross-reactions rate Diazepam 100% Nitrazepam 7.6% Oxazepam 8.8% 3. Components 1） Micro-well strips: 12 strips with 8 removable wells each 2） 6× standard solution (1 mL each): 0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb 3） Enzyme conjugate (12 mL) red cap 4） Antibody working solution (7 mL) blue cap 5） Substrate A solution (7 mL) white cap 6） Substrate B solution (7 mL) black cap 7） Stop solution (7 mL) yellow cap 8） 20× concentrated washing buffer (40 mL) white cap 9） 2× concentrated redissolving solution (50 mL) transparent cap 4. Materials required but not provided 1) Equipments:microplate reader, printer, homogenizer, nitrogen-drying device, votex, centrifuge, measuring pipets, balance (a sensibility reciprocal of 0.01 g). 2) Micropipettors: single-channel 20 to 100 µL and 200 to 1000 µL, and multi-channel 250 µL. 3) Reagents: N-hexane, NaOH,Dichloromethane(CH2Cl2), CH3CN.