Food safety test kit LSY-10001

for animalsfor nitrofuranedensity

This test kit is based on the competitive enzyme immunoassay for the detection of AMOZ in the sample. The coupling antigens are pre-coated on the micro-well stripes. The AMOZ in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-AMOZ antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the AMOZ in it. This value is compared to the standard curve and the AMOZ concentration is subsequently obtained. 2. Technical specifications Sensitivity: 0.03ppb Incubation Temperature: 25℃ Detection limit Tissue, egg, honey: 0.1ppb Cross-reaction rate AMOZ 100% AHD <0.1% AOZ <0.1% SEM <0.1% Recovery rate Tissue 80±25% Honey 75±25% Egg 95±25% 1） Equipments:microplate reader, printer, homogenizer, nitrogen-drying device, vortex, centrifuge, measuring pipets, balance (a reciprocal sensibility of 0.01 g), incubator, water bath; 2） Micropipettors: single-channel 20-200µL, 100-1000µL, and multi-channel 30～300µl; 3） Reagents: NaOH, ethyl acetate, n-Hexane, HCI (approx 36.5%), K2HPO4·3H2O 5. Sample pre-treatment Instructions The following points must be dealt with before the pre-treatment of any kind of sample: 1） Only the disposable tips can be used for the experiments and the tips must be changed when used for absorbing different reagents; 2） Before the experiment, each experimental equipment must be clean and should be re-cleaned if necessary, in order to avoid the contamination that interferes with the experimental results.