The Gentamicin, Kanamycin, Neomycin and Streptomycin combo rapid test strip is based on Colloidal gold immunochromatography, used for testing Gentamicin, Kanamycin, Neomycin and Streptomycin residue in milk(including pure milk and fresh milk) and goat milk qualitatively. The whole test only need 10min, east to operate with high sensitivity.
5. Principle
The Gentamicin, Kanamycin, Neomycin and Streptomycin combo rapid test strip is based on competitive inhibition immuno-chromatographic principle. In the flow process, antibiotics in the sample combined with antibiotics specific colloidal gold-labeled monoclonal antibody, Inhibit the combination between antibody and antibiotics-BSA conjugate on Test line of NC membrane, lead to the color change of Test line. When the concentration is equal to or higher than detection limit, T line is lighter than C line or T line has no color or T line has same color with C line.When the sample has no antibiotics residue or concentration lower than detection limit, T line is darker then C line; No matter whether there is antibiotics residue in sample, C line will appear, it means the test is valid.
6.Operation procedures
6.1 Read the instruction carefully before use. Return test strips and sample into room temperature.
6.2 Take bottles needed from the kit package, take out required microwells, making proper marks, Please use it in 1 hour. Seal the cap of the bottles, avoid moisture.
6.4 Incubate at (40±2℃) for first step, timing for 5min.