This Rapid Fecal Occult Blood (FOB) Test Kit (Colloidal Gold) is a rapid immunological test intended for the qualitative detection of fecal occult blood in feces. The test is intended for the determination of gastrointestinal (GI) bleeding, found in a number of gastrointestinal disorders, e.g., diverticulitis, colitis, polyps, and colorectal cancer. This FOB test is recommended for use in (1) routine physical examinations, (2) monitoring any bleeding in patients, and (3) screening for colorectal cancer or gastrointestinal bleeding.
Test Principle
The Rapid Fecal Occult Blood (FOB) Test Kit is a “sandwich” immunoassay utilizing two monoclonal antibodies to specifically detect the presence of human hemoglobin in feces. A stool specimen is collected into the sampling tube containing extraction solution. If human hemoglobin is present at a level higher than 100 ng/mL in a fecal sample extract, an immuno-complex of “labeled monoclonal anti-human hemoglobin Antibody – human hemoglobin – membrane coated monoclonal anti-human hemoglobin antibody” is formed. A red colored band appears in the test (T) region, a similar colored band must appear in the control (C) region, indicating the test strip is functioning properly and the result is valid.
Interpretation Of Results
• Positive:
If two colored bands (T and C lines) are visible within 5 minutes, the test result is positive and valid.
• Negative:
If there is no T line and Only the C line is displayed, the test resuit is negative.
• Invalid:
If there is no C line in the control area, regardless of any line in the test area or not, the test resuit is invalid.