Single-cell suspension dissociator PythoN™

The Singleron PythoN™ Automated Tissue Dissociation System integrates four dissociation steps: mechanical cutting, grinding, heating, and enzymatic dissociation. The streamlined workflow quickly dissociates the tissue into high-quality single-cells suspension. Equipped with broad-spectrum sCelLiVE™ Tissue Dissociation Solution and disposable PythoN Dissociation Tubes, the system can automatically process tissues to obtain highly viable and high-quality single-cell suspensions. The precise digital controling module enables a quick exploration of the best dissociation conditions for a specific tissue type. Highlights Efficient 8 samples in parallel. 15 min workflow Heating, mechanical dissociation and enzymatic reaction in one step Convenient Complété workflow, touch screen and start No shredding required for 100mg+ samples Flexible Dissociation solution suitable for 200+ tissue types Suitable for sample sizes starting from 10 mg Intelligent Simple interface, easy to operate Built-in preset programs, adjustable and storable In clinical settings, in order to detect the pathological characteristics of specific tissues, needle biopsy is often required. The low weight of the obtained needle biopsies is often a challenge for single-cell sequencing workflow (the weight of a single sample is about 20-40 mg). Here, we used Singleron PythoN® to process 14 mg and 33 mg mouse liver tissues, and achieved high cell viability and yield accordingly. This enabled applying clinical needle biopsies to single cell sequencing workflows.