Solution reagent kit CLindex™

for DNA library preparationfor RNA library preparation

CLindex™Sample multiplexing kit enables pooling of up to 16 sample in the same single cell sequencing library. The CLindex™ multiplexing technology employs click-chemistry for efficient, unbiased sample tagging. A chemical group with a high affinity to cell surface proteins and a sample indexing oligo binding to that chemical group label the cells of each sample. A major advantage of the click chemistry-based labeling compared to antibody-based labeling is that the cell surface proteins recognized by click chemistry are abundant and found across different species. Furthermore, the entire process of sample tagging takes only 30 min. Streamlined Workflow Sample tags are added using click chemistry prior to sample pooling in a single GEXSCOPE® workflow. Pooled cells with sample tags are then loaded to a SCOPE-chip™ for cell lysis and barcoding. The sample tag (15nt) is flanked by a universal PCR handle and a poly(A)-tail, which are captured by oligo-dT beads. Sample tags are labeled with a cell barcode. Simultaneously, mRNAs of the same single cell are also captured by the magnetic oligo-dT beads at their poly(A)-tail and tagged with the same cell barcode as well as UMI. Thus, all cDNA fragments from the same cell have a common denominator: the cell barcode. Both the cell barcode and the CLindex tag ensure that each transcript can be linked back to the cell and sample of origin. High Labeling efficiency Four independent cell lines (NB4, THP1, U937 and CCRF) were labeled using CLindex® and pooled in equal parts prior to cell partitioning on the SCOPE-chip®.